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The teamStaffSilvio Uhlig    October 23, 2018
Silvio Uhlig, Dr. scient.
 


Section for Chemistry
Norwegian Veterinary Institute
Ullevålsveien 68
0454 Oslo/Norway

Tel.: +47 2321 6264
Fax: +47 2321 6201
Skype: silvio.oslo
E-mail: silvio.uhlig@vetinst.no

 
 
Research interest

Chemistry of toxins from fungi, cyanobacteria, algae and plants; their isolation, purification and characterisation as well as biological effects. Instrumental analysis of small molecules including mass spectrometry and NMR spectroscopy. Metabolomics.

 
Background

Background in chemical engineering, organic and analytical chemistry from the University of Applied Sciences Dresden/Germany and University of Oslo/Norway. Professional experience from Zeneca Specialties Manchester/UK and Institute for Energy Technology Kjeller/Norway. Doctoral degree from the Norwegian Veterinary Institute/Norwegian School of Veterinary Science Oslo/Norway. Senior Researcher at the Norwegian Veterinary Institute since 2005, Head of Chemistry Section since 2016.

 
Original publications
 
    Handeland, K., Vikøren, T., Josefsen, T., Valdecanas, B., Uhlig, S. Yew (Taxus) intoxication in free-ranging cervids. PlosOne 2017, 12 (12), 1–10.
   Øya, E., Afanou, A.K.J., Malla, N, Uhlig, S., Rolen, E., Skaar, I., Straumfors, A., Winberg, J.O., Bang, B.E., Schwarze, P.E., Eduard, W., Holme, J.A. Characterization and pro-inflammatory responses of spore and hyphae samples from various mold species, submitted for publication in Particle and Fibre Toxicology 2017, 28, 28–39.
    Uhlig, S., Stanic, A., Hussain, F., Miles, C.O. Selectivity of commercial immunoaffinity columns for modified forms of the mycotoxin 4-deoxynivalenol (DON), Journal of Chromatography B 2017, 1061–1062, 322–326.
   Ivanova, L., Uhlig, S., Devreese, M., Croubels, S., Fæste, C.K. Biotransformation of the mycotoxin enniatin B1 in pigs: a comparative in vitro and in vivo approach, Food and Chemical Toxicology 2017, 105, 506–517.
   Moldes-Anaya, A., Sæther, T., Uhlig, S., Nebb, H.I., Larsen, T., Eilertsen, H.C., Paulsen, S.M. Two isomeric C16 oxo-fatty acids from the diatom Chaetoceros karianus show isoform-specific agonist activity towards human peroxisome proliferator-activated receptors (PPARs), Marine Drugs 2017, 15, 148–163.
   Ivanova, L., Sahlstrøm, S., Rud, I., Uhlig, S., Fæste, C.K., Eriksen, G.S., Divon, H. Effect of primary processing on the distribution of free and modified Fusarium mycotoxins in naturally contaminated oats, World Mycotoxin Journal 2017, 10, 73–88.
   Uhlig, S., Stanic, A., Hofgaard, I.S., Kluger, B., Schuhmacher, R., Miles, C.O. Glutathione-conjugates of deoxynivalenol in naturally contaminated grain are primarily linked via the epoxide group, Toxins 2016, 8, 329–340.
   Stanic, A., Uhlig, S., Sandvik, M., Rise, F., Wilkins, A., Miles, C.O. Characterization of deoxynivalenol–glutathione conjugates using nuclear magnetic resonance spectroscopy and liquid chromatography–high resolution mass spectrometry, Journal of Agricultural and Food Chemistry 2016, 64, 6903–6910.
   Pasquali, M., Beyer, M., Logrieco, A., Audenaert, K., Balmas, V., Basler, R., Boutigny, A.-L., Chrpova, J., Czembor, E., Gagkaeva, T., Gonzales-Jaen, M.T., Hofgaard, I.S., Köycü, N.D., Hoffmann, L., Levic, J., Marin, P., Miedaner, T., Migheli, Q., Moretti, A., Müller, M.E.H., Munaut, F., Parikka, P., Pallez-Barthel, M., Piec, J., Scauflaire, J., Scherm, B., Stankovic, S., Thrane, U., Uhlig, S., Vanheule, A., Yli-Mattila, T., Vogelgsang, S. A European database of Fusarium graminearum and Fusarium culmorum trichothecene genotypes, Frontiers in Microbiology 2016, 7, 406–416.
   Stanic, A., Uhlig, S., Solhaug, A., Rise, F., Wilkins, A., Miles, C.O. Preparation and characterization of cysteine adducts of deoxynivalenol, Journal of Agricultural and Food Chemistry 2016, 64, 4777–4785.
   Uhlig, S.; Ivanova, L. and Fæaste, C. K. Correction to Enzyme-assisted synthesis and structural characterization of the 3-, 8- and 15-glucuronides of deoxynivalenol, Journal of Agricultural and Food Chemistry 2016, 64, 3732.
   Frandsen, R.J.N., Rasmussen, S.A., Knudsen, P.B., Uhlig, S., Petersen, D., Lysøe, E., Gotfredsen, C.H., Giese, H., Larsen, T.O. Perithecial pigmentation in Fusarium species with black perithecia is due to the accumulation of 5-deoxybostrycoidin-based melanin, Scientific Reports 2016 6, 26206–26220.  
   Beccari, G.; Caproni, L.; Tini; F., Uhlig, S.; Covarelli, L. Presence of Fusarium species and other toxigenic fungi in malting barley and multi-mycotoxin analysis using liquid chromatography – high resolution mass spectrometry, Journal of Agricultural and Food Chemistry 2016, 64,4390–4399.
   Uhlig, S.; Negård, M.; Heldal, K.K.; Straumfors, A.; Madsø, B.; Bakke, B.; Eduard, W. Profiling of 3-hydroxy fatty acids as environmental markers of endotoxin using liquid chromatography coupled to tandem mass spectrometry. Journal of Chromatography A 2016, 1434, 119-126.   
    Huybrechts, B.; Martins, J.C.; Debongnie, P.; Uhlig, S.; Callebaut, A. Fast and sensitive LC–MS/MS method measuring human mycotoxin exposure using biomarkers in urine, Archives in Toxicology 2015, 89, 1993-2005.
    Frizzell, C.; Uhlig, S.; Miles, C.O.; Verhaegen, S.; Elliott, C.T.; Eriksen, G.S.; Sørlie, M.; Ropstad, E.; Connolly, L. Biotransformation of zearalenone and zearalenols to their major glucuronide metabolites reduces estrogenic activity, Toxicology In Vitro 2015, 29, 575-581.
    Stanic, A.; Uhlig, S.; Solhaug, A.; Rise, F.; Wilkins, A.; Miles, C.O. Nucleophilic addition of thiols to deoxynivalenol, Journal of Agricultural and Food Chemistry 2015, 63, 7556-7566.
    Aamot, H.U.; Ward, T.J.; Brodal, G.; Vrålstad, V.; Larsen, G.B.; Klemsdal, S.; Elameen, A.; Uhlig, S.; Hofgaard, I.S. Genetic and phenotypic diversity within the Fusarium graminearum species complex in Norway, European Journal of Plant Pathology 2015, 142, 501-519.   
    Negård, M.; Uhlig, S.; Kauserud, H.; Andersen, T.; Høiland, K.; Vrålstad, T. Indole-alkaloid profiles in Norwegian Claviceps purpurea populations - relationships between chemoraces, genetic groups and ecology, Toxins 2015, 7, 1431-1456.
    Straumfors, A.; Uhlig, S.; Eriksen, G.S.; Heldal, K.K.; Eduard, W.; Krska, R.; Sulyok, M. Mycotoxins and other fungal metabolites in grain dust from Norwegian grain elevators and compound feed mills. World Mycotoxin Journal 2015, 8, 361-373.
    Uhlig, S.; Hussain, F.; Wisløff, H. Bioassay-guided fractionation of extracts from Easter lily (Lilium longiflorum) flowers reveals unprecedented structural variability of steroidal glycoalkaloids. Toxicon 2014, 92, 42-49.
    Uhlig, S.; Egge-Jacobsen, W.; Vrålstad, T.; Miles, C.O. Indole–diterpenoid profiles of Claviceps paspali and Claviceps purpurea from high-resolution Fourier transform Orbitrap mass spectrometry. Rapid Communications in Mass Spectrometry 2014, 28, 1621-1634.   
    Uhlig, S.; Eriksen, G.S.; Hofgaard, I.S.; Krska, R.; Beltran, E.; Sulyok, M. Faces of a changing climate: Semi-quantitative multi-mycotoxin analysis of grain grown in exceptional climatic conditions in Norway. Toxins 2013, 5, 1682-1697. open access
    Ivanova, L.; Fæste, C.K.; Delezie, E.; Van Pamel, E.; Daeseleire, E.; Callebaut, A.; Uhlig, S. Presence of enniatin B and its hepatic metabolites in plasma and liver samples from broilers and eggs from laying hens. World Mycotoxin Journal 2014, 7, 167-175.
    Sarkanj, B.; Warth, B.; Uhlig, S.; Abia, W.A.; Sulyok, M.; Klapec, T.; Krska, R.; Banjari, I. Urinary analysis reveals high deoxynivalenol exposure in pregnant women from Croatia. Food and Chemical Toxicology 2013, 62, 231-237.
    Uhlig, S.; Ivanova, L.; Fæste, C.K. Enzyme-assisted synthesis and structural characterization of the 3‑, 8‑, and 15-glucuronides of deoxynivalenol. Journal of Agricultural and Food Chemistry 2013, 61, 2006-2012.   
    Uhlig, S.; Busman, M.; Shane, D. S.; Rønning, H.; Rise, F.; Proctor, R. Identification of early fumonisin biosynthetic intermediates by inactivation of the FUM6 gene in Fusarium verticillioides. Journal of Agricultural and Food Chemistry 2012, 60, 10293-10301.
    Santini, A.; Meca, G.; Uhlig, S.; Ritieni, A. Fusaproliferin, beauvericin and enniatins: Occurrence in food - a review, World Mycotoxin Journal 2012, 5, 71-81.
    Busk, Ø. L.; Frizzell, C.; Verhaegen; S.; Uhlig, S.;  Connolly, L.; Ropstad, E.; Sørlie, M. Cytosol protein regulation in H295R steroidogenesis model induced by the zearalenone metabolites, α-and β-zearalenol. Toxicon 2012, 59, 17-24.
    Uhlig, S.; Kaldhusdal, M.; Gjevre, A.-G. LC-MS-MS determination of gizzerosine in poultry feed. Chromatographia 2011, 74, 133-138.
    Fæste, C. K.; Ivanova, L.; Uhlig, S. In vitro metabolism of the mycotoxin enniatin B in different species and CYP P450 reaction phenotyping. Drug Metabolism and Disposition 2011, 39, 1768-1776.
    Ivanova, L.; Fæste, C. K.; Uhlig, S. In vitro metabolism of the depsipeptide enniatin B. Analytical and Bioanalytical Chemistry 2011, 400, 2889-2901.
    Uhlig, S.; Petersen, D.; Rolen, E.; Egge-Jacobsen, W.; Vrålstad, T. Ergosedmine, a new peptide ergot alkaloid from the ergot fungus, Claviceps purpurea, parasitizing Calamagrostis arundinacea.  Phytochemistry Letters 2011, 4, 79-85.
    Moldes-Anaya, A.; Rundberget, T.; Uhlig, S.; Rise, F.; Wilkins, A. L. Isolation and structure elucidation of secopenitrem D, an indole alkaloid from Penicillium crustosum Thom.  Toxicon 2011, 57  (2), 259-265. Abstract
    Ivanova, L.; Uhlig, S.; Eriksen, G. S.; Johannessen, L. E. Enniatin B1 is a substrate of intestinal P-glycoprotein, multidrug resistance-associated protein 2 and breast cancer resistance protein. World Mycotoxin Journal 2010, 3 (3), 271-281.
    Ivanova, L.; Petersen, D.; Uhlig, S. Phomenins and fatty acids from Alternaria infectoria. Toxicon 2010, 55 (6), 1107-1114.
    Langseth, W.; Rundberget, T.; Uhlig, S. Electron ionisation mass spectrometry of the pentafluoropropionate esters of trichothecene analogues and culmorin compounds from Fusarium species. Rapid Communications in Mass Spectrometry 2010, 24 (17), 2641-2649.
    Jestoi, M.; Kokkonen, M.; Uhlig, S. What about the 'other' Fusarium mycotoxins? World Mycotoxin Journal 2009, 2 (2), 181-192.
    Uhlig, S.; Ivanova, L.; Petersen, D.; Kristensen, R. Structural studies on minor enniatins from Fusarium sp VI 03441: Novel N-methyl-threonine containing enniatins. Toxicon 2009,  53 (7-8), 734-742.
    Uhlig, S.; Botha, C. J.; Vrålstad, T.; Rolen, E.; Miles, C. O. Indole-diterpenes and ergot alkaloids in Cynodon dactylon (Bermuda Grass) infected with Claviceps cynodontis from an outbreak of tremors in cattle. Journal of Agricultural and Food Chemistry 2009, 57 (23), 11112-11119.
    Ivanova, L.; Uhlig, S. A bioassay for the simultaneous measurement of metabolic activity, membrane integrity, and lysosomal activity in cell cultures. Analytical Biochemistry 2008, 379 (1), 16-19.
    Uhlig, S.; Ivanova, L.; Bernhoft, A.; Eriksen, G. S. 2-Amino-14,16-dimethyloctadecan-3-ol: in vitro bioactivity and bio-production by the fungus Fusarium avenaceum. World Mycotoxin Journal 2008, 1 (1), 49-58.
    Uhlig, S.; Petersen, D. Lactam ergot alkaloids (ergopeptams) as predominant alkaloids in sclerotia of Claviceps purpurea from Norwegian wild grasses. Toxicon 2008, 52 (1), 175-185.
    Wisløff, H.; Uhlig, S.; Scheie, E.; Loader, J.; Wilkins, A.; Flåøyen, A. Toxicity testing of saponin-containing Yucca schidigera Roetzl. juice in relation to hepato- and nephrotoxicity of Narthecium ossifragum (L.) Huds. Toxicon 2008, 51 (1), 140-150.
    Uhlig, S.; Wisløff, H.; Petersen, D. Identification of cytotoxic constituents of Narthecium ossifragum using bioassay-guided fractionation. Journal of Agricultural and Food Chemistry 2007, 55 (15), 6018-6026.
    Uhlig, S.; Jestoi, M.; Parikka, P. Fusarium avenaceum -- the North European situation. International Journal of Food Microbiology 2007, 119 (1-2), 17-24.
    Uhlig, S.; Vikoren, T.; Ivanova, L.; Handeland, K. Ergot alkaloids in Norwegian wild grasses: a mass spectrometric approach. Rapid Communications in Mass Spectrometry 2007, 21 (10), 1651-1660.
    Ivanova, L.; Skjerve, E.; Eriksen, G. S.; Uhlig, S. Cytotoxicity of enniatins A, A1, B, B1, B2 and B3 from Fusarium avenaceum. Toxicon 2006, 47 (8), 868-876.
    Uhlig, S.; Jestoi, M.; Kristin, K. A.; Heier, B. T. Multiple regression analysis as a tool for the identification of relations between semi-quantitative LC-MS data and cytotoxicity of extracts of the fungus Fusarium avenaceum (syn. F. arthrosporioides). Toxicon 2006, 48 (5), 567-579.
    Uhlig, S.; Torp, M.; Heier, B. T. Beauvericin and enniatins A, A1, B and B1 in Norwegian grain: a survey. Food Chemistry 2006, 94 (2), 193-201.
    Uhlig, S.; Petersen, D.; Flaoyen, A.; Wilkins, A. 2-Amino-14,16-dimethyloctadecan-3-ol, a new sphingosine analogue toxin in the fungal genus Fusarium. Toxicon 2005,  46 (5), 513-522.
    Uhlig, S.; Gutleb, A. C.; Thrane, U.; Flåøyen, A. Identification of cytotoxic principles from Fusarium avenaceum using bioassay-guided fractionation. Toxicon  2005, 46 (2), 150-159.
    Uhlig, S.; Torp, M.; Jarp, J.; Parich, A.; Gutleb, A. C.; Krska, R. Moniliformin in Norwegian grain. Food Additives and Contaminants 2004, 21 (6), 598-606.
    Uhlig, S.; Ivanova, L. Determination of beauvericin and four other enniatins in grain by liquid chromatography-mass spectrometry. Journal of Chromatography A 2004, 1050 (2), 173-178.
    Morrison, E.; Kosiak, B.; Ritieni, A.; Aastveit, A. H.; Uhlig, S.; Bernhoft, A. Mycotoxin production by Fusarium avenaceum strains isolated from Norwegian grain and the cytotoxicity of rice culture extracts to porcine kidney epithelial cells. Journal of Agricultural and Food Chemistry 2002, 50 (10), 3070-3075.
 
 
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